Volume 5.11 | Mar 27

Extracellular Matrix News 5.11 March 27, 2014
Extracellular Matrix News
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A Time- and Matrix-Dependent TGFBR3-JUND-KRT5 Regulatory Circuit in Single Breast Epithelial Cells and Basal-Like Premalignancies
In an immortalized basal-like breast epithelial cell line, the authors identified two anticorrelated gene-expression programs that arise among single extracellular matrix-attached cells during organotypic three-dimensional culture. [Nat Cell Bio] Abstract
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PUBLICATIONS (Ranked by impact factor of the journal)
Spatiotemporally Controllable and Cytocompatible Approach Builds 3D Cell Culture Matrix by Photo-Uncaged-Thiol Michael Addition Reaction
A new concept of photocrosslinking mechanism is developed to construct a 3D cell culture matrix based on a photo-uncaged-thiol Michael addition. [Adv Mater] Abstract

The Chemokine Receptor CXCR6 Contributes to Recruitment of Bone Marrow-Derived Fibroblast Precursors in Renal Fibrosis
As chemokines mediate the trafficking of circulating cells to sites of injury, scientists studied the role of CXCR6 in mouse models of renal injury. Significantly, the kidney of CXCR6 knockout mice accumulated fewer bone marrow-derived fibroblasts in response to injury, expressed less profibrotic chemokines and cytokines, displayed fewer myofibroblasts, and expressed less α-smooth muscle actin in the obstructed kidneys compared with wild-type mice. [Kidney Int] Abstract

Strain-Dependent Modulation of Macrophage Polarization within Scaffolds
Implanted synthetic substrates for the regeneration of cardiovascular tissues are exposed to mechanical forces that induce local deformation. Circulating inflammatory cells, actively participating in the healing process, will be subjected to strain once recruited. The authors investigated the effect of deformation on human peripheral blood mononuclear cells adherent onto a scaffold, with respect to macrophage polarization towards an inflammatory and reparative phenotype and to early tissue formation. [Biomaterials] Abstract

Stringent Requirement for Spatial Arrangement of Extracellular Matrix in Supporting Cell Morphogenesis and Differentiation
Investigators found that tendon sections can be viewed as a pre-formed block of extracellular matrix in which the collagen fibrils exhibited a spatial regularity unraveled in any artificially constructed scaffold. By carving the tendon at different angles relative to its main axis, they created different surfaces with distinct spatial arrangements of collagen fibrils. [BMC Cell Biol] Abstract | Full Article

MiR-29b Inhibits Collagen Maturation in Hepatic Stellate Cells through Down-Regulating the Expression of HSP47 and Lysyl Oxidase
Heat shock protein 47 (HSP47) and lysyl oxidase (LOX) are known to be essential for extracellular matrix (ECM) maturation. Scientists demonstrated that expression of HSP47 and LOX was significantly up-regulated in culture-activated primary rat hepatic stellate cells, TGF-β stimulated LX-2 cells and liver tissue of CCl4-treated mice, which was accompanied by a decrease of miR-29b level. [Biochem Biophys Res Commun] Abstract

Comparative Performance of Collagen Nanofibers Electrospun from Different Solvents and Stabilized by Different Crosslinkers
Collagen type I was successfully electrospun from two different solvents, trifluoroethanol and dilute acetic acid. Characterization of collagen fibers was performed by means of SEM, ATR-IR, Circular Dichroism and WAXD. [J Mater Sci Mater Med] Abstract

The Influence of Selected Flavonoids from the Leaves of Cirsium palustre (L.) Scop. on Collagen Expression in Human Skin Fibroblasts
Ten flavonoids belonging to the subclasses of flavones, flavanones and aurones were isolated from methanolic extract of Cirsium palustre leaves after multistep chromatographic separation. Four compounds-eriodictyol 7-O-glucoside, 6-hydroxyluteolin 7-O-glucoside, scutellarein 7-O-glucoside and pedalitin-were tested for their effect on collagen expression in normal human dermal fibroblasts. [Phytother Res] Abstract

Effects of Various Extracellular Matrix Proteins on the Growth of HL-1 Cardiomyocytes
Researchers present the physical and biochemical effects of extracellular matrices (ECMs) on HL-1 cardiomyocytes. ECMs play major roles in cell growth, adhesion and the maintenance of native cell functions. [Cells Tissues Organs] Abstract

Take the if out of diff: Reduce variability when differentiating to definitive endoderm
Matricellular Proteins and Biomaterials
Expression of matricellular proteins is modulated during the foreign body response and these proteins interact with biomaterials. The design of biomaterials to specifically alter the levels of matricellular proteins surrounding implants provides a new avenue for the design and fabrication of biomimetic biomaterials. [Matrix Biol]
Full Article

Visit our reviews page to see a complete list of reviews in the extracellular matrix research field.
NUI Galway Researchers Encouraged by Positive Results on New Osteoarthritis Treatment
National University of Ireland, Galway (NUI Galway) researchers are part of an exciting EU-funded project looking at how stem cells from adult fat tissue could be used to activate the regeneration of cartilage. If successful, their work could lead to effective new treatments for millions of osteoarthritis sufferers. [National University of Ireland, Galway] Press Release

NeoStem Begins Research Collaboration for Treatment of Skin Wounds in Scleroderma
NeoStem, Inc. announced that it has signed an agreement for a research collaboration investigating the potential of very small embryonic-like stem cells in treating difficult to heal wounds in an animal model of scleroderma. [NeoStem, Inc.] Press Release

Kazakh Scientists Trained on Latest Technology
Five scientists from Kazakhstan are being trained on cutting-edge stem cell and genomic technology at the Buck Institute, in a Kazakhstan Ministry of Health-funded project aimed at designing novel therapeutics to treat skin wounds caused by diabetes. [Buck Institute] Press Release

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NEW BIT 7th Annual World Congress of Regenerative Medicine & Stem Cells 2014
November 13-16, 2014
Haikou, China

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NEW Postdoctoral Position – The Extracellular Matrix in Aging, Exercise and in the Tumor Microenvironment (UFSCar)

NEW Postdoctoral Position – Use of Scaffold and Cells for Orthopaedic Applications (Bone Therapeutics S.A.)

Postdoctoral Position – Tumor Microenvironment (Baylor College of Medicine)

Director of GMP/GLP Quality Operations (University of Pennsylvania, Perelman School of Medicine)

PhD Position – 3D Structured Self-Assembled Hydrogels for Tissue Engineering (TU Delft)

Faculty Position – Cervical Biology (Wayne State University School of Medicine)

Postdoctoral Research Fellow – Mechanobiology, Fibroblasts and Matrix Biology (Mayo Clinic)

Scientific Communications & Publishing Coordinator (STEMCELL Technologies, Inc.)

Research Technologist – Pluripotent Stem Cells (STEMCELL Technologies Inc.)

Research Technologist – Particle Chemistry (STEMCELL Technologies Inc.)

Research Technologist – PSC Biology and Bioengineering (STEMCELL Technologies Inc.)

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