Volume 4.31 | Aug 8

Extracellular Matrix News 4.31 August 8, 2013
Extracellular Matrix News
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CCM1-ICAP-1 Complex Controls β1 Integrin-Dependent Endothelial Contractility and Fibronectin Remodeling
Scientists demonstrated that cerebral cavernous malformation (CCM)1/2 loss resulted in ICAP-1 destabilization, which increased β1 integrin activation and led to increased RhoA-dependent contractility. The resulting abnormal distribution of forces led to aberrant extracellular matrix remodeling around lesions of CCM1- and CCM2-deficient mice. [J Cell Biol] Abstract
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PUBLICATIONS (Ranked by impact factor of the journal)
Granzyme B Degrades Extracellular Matrix and Contributes to Delayed Wound Closure in Apolipoprotein E Knockout Mice
The authors used apolipoprotein E knockout mice as a novel model of chronic inflammation and impaired wound healing to investigate the role of granzyme B in chronic wounds. [Cell Death Differ] Abstract

Fibronectin Expression in Glioblastomas Promotes Cell Cohesion, Collective Invasion of Basement Membrane In Vitro and Orthotopic Tumor Growth in Mice
Scientists sought to examine the expression of tumor cell fibronectin and address its possible involvement in the invasive phenotype of glioblastoma multiforme. [Oncogene] Abstract

Urine Proteomes of Healthy Aging Humans Reveal Extracellular Matrix (ECM) Alterations and Immune System Dysfunction
Urine samples from 37 healthy volunteers aged 19–90 years were analyzed for protein expression by liquid chromatography-tandem mass spectrometry. This generated a list of 19 proteins that were differentially expressed in different age groups. In particular, the oldest group showed protein changes reflective of altered ECM turnover and declining immune function, in which changes corresponded to reported changes in cardiovascular tissue remodeling and immune disorders in the elderly. [Age] Abstract

Connective Tissue Growth Factor Induces Collagen I Expression in Human Lung Fibroblasts through the Rac1/MLK3/JNK/AP-1 Pathway
Scientists investigated the role of Rac1, mixed-lineage kinase 3 (MLK3), c-Jun N-terminal kinase (JNK), and activator protein-1 (AP-1) in connective tissue growth factor-induced collagen I expression in human lung fibroblasts. [Biochim Biophys Acta] Abstract

Serine-Threonine Kinase Receptor Associated Protein (STRAP) Regulates Translation of Type I Collagen mRNAs
The authors showed that STRAP is tethered to collagen mRNAs by interaction with LARP6. LARP6 is a protein which directly binds the 5’ stem-loop (5’SL) present in collagen α1(I) and α2(I) mRNAs, but it interacts with STRAP with its C-Terminal domain which is not involved in binding 5’SL. [Mol Cell Biol] Abstract

Development of an In Vitro Cell System from Zebrafish Suitable to Study Bone Cell Differentiation and Extracellular Matrix Mineralization
Mechanisms of bone formation and skeletal development have been successfully investigated in zebrafish using a variety of in vivo approaches, but in vitro studies have been hindered due to a lack of homologous cell lines capable of producing an extracellular matrix suitable for mineral deposition. Here investigators describe the development and characterization of a new cell line termed ZFB1, derived from zebrafish calcified tissues. [Zebrafish] Abstract

Cell Surface Sialic Acid Modulates Extracellular Matrix Adhesion and Migration in Pancreatic Adenocarcinoma Cells
Tumor cells modulate their extracellular matrix (ECM) adhesion and migration to become more metastatic. Moreover, they show an increase in sialic acid, which could have an effect on their ECM adhesion and migration. Scientists describe the influence of pancreatic adenocarcinoma cell surface [alpha]2,3- and [alpha]2,6-sialic acid determinants on the aforementioned processes. [Pancreas] Abstract

Optimization and Use of Humanized RBL NF-AT-GFP and NF-AT-DsRed Reporter Cell Lines Suitable for High-Throughput Scale Detection of Allergic Sensitisation in Array Format and Identification of the ECM-Integrin Interaction as Critical Factor
The authors previously described a microarray platform combining live basophils with protein arrays suitable for high-throughput detection of sensitization against allergens. During optimization of this technique, they observed severe losses of adhering cells during the washing steps, particularly after activation. In order to preserve cell binding, they tested the cell adhesion characteristics of different extracellular matrix (ECM) proteins: human collagen I, fibronectin from bovine plasma and laminin. [Mol Biotechnol] Full Article

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Role of MicroRNA-Mediated MMP Regulation in the Treatment and Diagnosis of Malignant Tumors
In this review, the authors highlight the complicated interactive network comprised of different matrix metalloproteinases (MMPs) and their regulating microRNAs, as well as the ways in which these interactions influence cancer development, including tumor angiogenesis, growth, invasion, and metastasis. [Cancer Biol Ther] Abstract

Visit our reviews page to see a complete list of reviews in the extracellular matrix research field.
CorMatrix® Cardiovascular, Inc. Receives Three U.S. Patents for Biomaterial Heart Valve
CorMatrix® Cardiovascular, Inc. announced the receipt of three U.S. Patents for replacement heart valves that utilize unique biomaterial called extracellular matrix (ECM®). CorMatrix® has more than 120 U.S. and international patent applications pending on various ECM-based compositions and structures as well as methods for processing ECM® materials and forming the ECM® structures. [Business Wire] Press Release

Grant to Support Commercialization of Technology to Repair Skin Injuries
The Chemical Alliance Zone’s Chemicals and Materials Commercialization Fund has awarded $20,000 to a Marshall University scientist to help bring to market a technology he has developed for repairing skin injuries. The award to Dr. Jingwei Xie of the Marshall Institute for Interdisciplinary Research will help Xie’s research team produce device prototypes and samples, market to potential customers and conduct patent analysis. [Marshall University] Press Release

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NEW Second Annual Engineering Functional 3-D Tissue Models
October 28-29, 2013
Cambridge, United States

Visit our events page to see a complete list of events in the extracellular matrix community.
NEW PhD Student – Coronary Artery Research (Erasmus MC)

PhD Position – Molecular Biomechanics of α-Actin Remodeling (Eindhoven University of Technology)

PhD Position – Biomaterials for Regenerative Medicine (Institute for Bioengineering of Catalonia)

PhD Position – Molecular Cell Biology (Weizmann Institute of Science)

Postdoctoral Fellow – Mechanisms Contributing to Metastases of Breast Cancer Cells (Mount Sinai Medical Center/Icahn School of Medicine at Mount Sinai)

Assistant/Associate Professor Position – Correlative Light and Electron Microscopy (Universite de Montreal)

Director of Cell Processing Facility (S L Collins Associates, Inc.)

PhD Position – 3D Structured Self-Assembled Hydrogels for Tissue Engineering (Delft University of Technology)

Postdoctoral Position – Tissue Engineering (Weizmann Institute of Science)

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